Title: TGF-β induces AP-1 DNA binding activity and promotes the expression of collagen mRNA in NIH3T3 cell
Abstract: Objective To investigate the effect of AP 1 on TGF β promoting the expression of typeα2Ⅰcollagen mRNA.Methods NIH3T3 cells were treated with TGF β,and nuclear proteins were extracted,then AP 1 DNA binding activity were analyzed by EMSA; Total RNA was isolated and analyzed by RT PCR.Results AP 1 DNA binding activity could be observed after the cells were treated with TGF β for 0.5 h,and the pick point was at 6 h,and then AP 1 activity decreased greadually,but AP 1 activity could be detected at 24th h; After the cells were treated with TGF β for 24 h,the expression of α2Ⅰ collagen mRNA was increased significantly.Conclusion Not only could TGF β induce AP 1 DNA binding activity,but also it could promote the expression of type α2Ⅰcollagen mRNA relative to untreated control.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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