Title: Analysis on drug resistance of human immunodeficiency virus by means of gene detection
Abstract: Objective:To analyze the HIV drug resistance in HIV suffers in the locality.Methods: Reverse transcription polymerase chain reaction(RT-PCR) technique was used to amplify the partial HIV-1 pol gene and detect their base sequences.The sequences phylogenesis were analyzed by landing the website http://hivdb.stanford.edu to know the drug resistance of HIV.Results: HIV load of 93 HIV-1/AIDS patients were more than 1000 cp/ml.The result of the genotype detection indicated that HIV-1 gene in 46 cases was amplificated successfully,including 10 untreated patients and 36 treated patients,and their HIV-1 subtype distribution contained CRF01-AE type(4 cases) and B type(42 cases).They were all drug resistance.The rate of drug resistance was 60.86%(28/46).Drug-resistance mutation,such as Q58E,M184V,Y181C,K103N,could result in decline or invalidation of PIs,NRTIs and NNRTIs′curative effect.Conclusion: Antivirus therapy could inhibit HIV-1 compication and improve the therapy effect.But the occurrence of drug resistance prompted that we must monitor HIV drug resistance in time and improve therapeutic schedule in order to lower the emergence and prevalence of drug-resistance virus.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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