Title: Analysis of Polysaccharide Composition of Marine Microalgae by Gas Chromatography
Abstract: The ultrasonication associated with extraction in. distilled water was used to extract polysaccha-ride in Chlorella. The crude polysaccharide was treated with a series of procedures of protein-removing, purification with column, hydrolysis and acetylation, then was analysized by Gas Chromatography to gain the saccharide components which are rhamnose、 ribose、 arabinose、 xylose、 mannose、 glucose and galacose and their percentage are 3. 0% ,1. 9% ,1. 3% ,1. 3%,1. 5% ,82. 8%and8. 2% respectively. The experimental result shows that the precision of the analytical method is good, with a relative standard deviation of 2. 1%-6. 4%. The recovery of the method is 96. 2%-102. 1%, and the detection limit is 0.002 38 μg.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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Cited By Count: 5
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