Title: Differences in the Accumulation of Some Organic Solutes and Gene Expression in Leaves Between the Salt tolerant Mutant and the Wild Type of Wheat Under NaCl Stress
Abstract: Proline content in leaves of the mutant increased more significantly than that of the wild type when exposed to NaCl stress (Fig.1). The content of proline increased for 72 h and then decreased in both types, but was higher in the mutant than in the wild type (Fig.2).When exposed to NaCl stress of 200 or 300 mmol/L for 96 h, the content of soluble sugars in leaves of the mutant maintained a higher level than that of the group without NaCl treatment, but that in the wild type became much lower than that of its control group (Fig.3). These results indicated that the contents of proline and soluble sugars in leaves of the mutant were higher than those of the wild type, which played the role of osmotic adjustment.The protein compositions of leaf cells of the mutant and the wild type were different. Under salt stress, the 88 kD peptide disappeared and an increase in 56 kD peptide was induced in leaf cells of the mutant by salt. In contrast, the quantity of the 88 kD peptide remained stable while the 56 kD peptide became less or even disappeared with the increase in salt concentration (Fig.4). By using DD PCR techniques, it was found that the mutant had six cDNA fragments specially expressed more than the wild type under salt stress (Fig.5).
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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Cited By Count: 1
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