Title: Research on Nucleus Distribution of Poria cocos and its Staining Technique
Abstract: Poria cocos originated from different regions being taken as materials,the mycelia growth situation,nucleus distribution and clamp connection were observed through the application of staining techniques such as Giemsa,Safranin O-KOH,Crystal violet,Basic carbolfuchsin and Hoechest 33258 in this study.The results showed that Giemsa and Hoechest 33258 staining methods exhibited the best staining efficiency,better one was Safranin O-KOH staining and last one was Crystal violet and Bacic carbolfuchsin staining.Multinucleate of Poria cocos could be observed under the fluorochromy microscope via Hoechest 33258 staining,but it was difficult to observe the existence of clamp connection.Under the light microscope,the septum and branching can be clearly observed in all above staining techniques,but the multinucleate of the Poria cocos could be clearly seen only in Giemsa staining.At the same time,the effectiveness of the same staining technique in different times was compared in this experiment.The result showed that Giemsa and Hoechest 33258 staining were the best in three minutes,Safranin O-KOH method was the best in five minutes staining and Crystal violet staining only needed one minute,but Bacic carbolfuchsin staining needed nine minutes.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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