Title: Effect of SB203580 on cell apoptosis during lung ischemic/reperfusion injury in rats
Abstract: AIM:To explore the effect of SB203580,P38 mitogen activated protein kinase specific inhibitor(p38MAPK),on apoptosis during lung ischemic/reperfusion injury in rats.METHODS:30 rats were randomly divided into three groups,group control(Group C),group lung ischemia/reperfusion injury(Group I/R)and group SB203580(Group S).The changes of arteriae pulmonalis apoptosis were detected with TdT-mediated dUTP nick end labeling(TUNEL).The content of Bcl-2 and Bax in cytoplasm were determined with immunohistochemistry and meanwhile the ultrastruction changes of lung were observed under electron microscope.RESULTS:Compared with Group C,the expressions of Bcl-2,Bax protein were increased and the ratio of Bcl-2/Bax were decreased in Group I/R(all P0.01),and the apoptosis index(AI)was increased,the abnormal changes of the lung tissue in morphologically were significant in Group I/R.After using SB203580,the expression of Bcl-2 protein was increased and the expression of Bax protein was decreased,the ratio of Bcl-2/Bax was increased(all P0.01),the values of AI was decreased in Group I/R,and the abnormal changes of the lung tissue in morphologically were lessen at different degree(all P0.01).CONCLUSION:SB203580 produces notable protective effects on lung ischemic/reperfusion injury in rats by up-regulating Bcl-2 protein expression,down-regulating Bax protein expression in lung tissue and regulating the balance of Bcl-2 protein and Bax protein,decreasing apoptosis.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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