Title: Adenosine A_1 Receptor Agonist Prevents Angiotensin II Induced Hypertrophy in Cultured Myocardial Cells of Neonatal Rats
Abstract: Objective To demonstrate the inhibitory effect of adenosine A1 receptor agonist R(-)-N6-(2-phenylisopropy1)adenosine(R-PIA)and cross-talk between adenosine A1 receptor and CaN in cultured myocardial cells hypertrophy induced by Ang Ⅱ.Methods The protein synthetic rate was determined by measuring the incorporation of [3H]-leucine into myocyte protein;the cardiomyocytes volumes were measured by computer photograph analysis system;the expression of atrial natriuretic polypeptide(ANP)and CaN were determined by Western-blot.Results R-PIA(1 μmol/L)inhibited Ang Ⅱ(0.1 μmol/L)-induced increase of [3H]-leucine incorporation [R-PIA:(976.2±89.0)vs Ang Ⅱ:(1130.7±102.7)count per min per well,P0.05] along with decreased the cardiomyocytes volumes [R-PIA:(1448±145)vs Ang Ⅱ:(2306±163)μm3,P0.05] and the expression of ANP,CaN.These responses were completely abolished by adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(CPDPX).Conclusion Adenosine A1 receptor stimulation significantly inhibits Ang Ⅱ-induced cardiac hypertrophy which may be partially by mediating CaN pathway.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot