Title: Extraction of soy isoflavone by ethanol/K_2 HPO_4 aqueous two-phase system
Abstract: The ethanol / K2HPO4 aqueous two-phase system is used to extract soy isoflavone from crude solid. On the basis of four factors and four levels orthogonal test,the effects of mass fraction of ethanol,mass fraction of K2HPO4,temperature and extracting time on the distribution coefficient and extraction yield of soy isoflavone are studied. The factors that influence the yield of soybean isoflavones are: extracting time mass fraction of K2HPO4 mass fraction of ethanol temperature. The optimal condition is obtained as follows: ethanol / K2HPO4 aqueous two-phase system( 25%and 25% of ethanol and K2HPO4,respectively) and extracting at 20℃ for 30 min. The soy isoflavone is almost assigned in the top phase. And the extraction yield of soy isoflavone in the top phase can reach 97. 0%.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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