Title: Study of Differentially Expressed Genes in Pancreatic Cancer by cDNA Microarray
Abstract: Objective To screen for the differentially expressed genes in pancreatic carcinoma and normal pancreatic tissue using cDNA microarray. Methods The PCR products of 4096 genes were spotted on a chemical-material-coated-glass plates in array. The DNAs were then fixed on the glass plate by a serial of treatments. The total RNAs were isolated from the tissues, and then mRNAs were purified to by Oligotex. Both mRNAs from pancreatic carcinoma and normal pancreatic tissue were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. After washing, the cDNA microarray was scanned. Results Among the 4 096 target genes, there were 949( 23.2% ) genes whose expression levels differed between the carcinoma and normal tissues. We identified 9 upregulated and 8 downregulated genes belonging to oncogenes and anti-oncogenes. Conclusion cDNA microarray technology is a powerful method in screening for differentially expressed genes. Further analysis of the obtained genes will help to understand the molecular mechanism of pancreatic carcinoma.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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