Title: Study on simultaneous determination for 22 veterinary drug residues in animal derived food by high performance liquid chromatography
Abstract:Objective: To develop a method based on high performance liquid chromatography(HPLC) for simultaneous determining residues of 22 veterinary drugs including Sulfanilamide,Sulfacetamide,Sulfathiazole,Su...Objective: To develop a method based on high performance liquid chromatography(HPLC) for simultaneous determining residues of 22 veterinary drugs including Sulfanilamide,Sulfacetamide,Sulfathiazole,Sulfadiazine,Sulfapyridine,Sulfachlorpyridazine,Sulfamerazine,Sulfamethazine,Olaquindox,Furazolidone,Chloramphenicol,Sulfisomezol,Sulfamethizole,Sulfamethoxydiazine,Diaveridine,Trimethopim,Sulfisoxazole,Sulfamethoxypyridazine,Sulfamonomethoxine Sodium,Sulfabenzamide,Sulfadimethoxine and Sulfaquinoxaline in animal derived food(livestock,poultry,aquatic products).Methods:Optimization of HPLC conditions for simultaneous determining 22 veterinary drugs: column Discovery C18,gradient and speed elution,UV detection at 270 nm.Samples are extracted with acetonitrile-chloroform(10+1),defatted with n-hexane,anti-extracted veterinary drugs with acetonitrile-chloroform(1+2),dissolved with mobile phase,analysised by HPLC.Results:Under the optimized HPLC conditions,separation of 22 veterinary drugs are better,linear ranges are wide,and good linear relationship(r=0.99~0.9999) in their concentration scope.Three levels of concentration are added to livestock,poultry,and aquatic product samples,the average recovery are 61.2%~103.0%,coefficient of variation was 1.0%~9.5%,the minimum detectable concentration are 0.004~0.018 mg/kg.Conclusion:This method is of accuracy,high repeatability,precision and less interferences from impurities,simple and fast.The cost is low and apply scope is wide.This method has met the requirement of analysis of veterinary drug multi-residues.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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