Title: Effects of Astragalus Injection on Myocardial Cell Damages Due to Oxidative Stress
Abstract: Objective To investigate the protective effects of Astragalus injection(AI)on hydrogen peroxide(H2O2)induced injury in cardiomyocytes.MethodsCultured neonatal rat cardiomyocytes were divided into:control group;H2O2 group,in which cells were treated with H2O2 0.15 mmol/L for 5 h;AI+H2O2 group,in which cells were pretreated with AI(with final concentration of 10,30,90 g/L)30 min before H2O2 treatment;and AI(90 g/L)+L-NAME(20 μg/L).The cardiomyocyte viability was analysed by MTT assay,lactate dehydrogenase(LDH)activity and nitric oxide(NO)content were detected in culture media.Reactive oxygen species(ROS)were measured with laser-confocal-microscopy system.Mitochondrial memberane potential(ΔΨm)and apoptosis rate were measured with flowcytometry.ResultsCardiomyocyte viability in AI(10,30,90 g/L)groups were higher than that in H2O2 group(P0.05).Compared with H2O2 group,LDH activity and ROS content in AI(90 g/L)group decreased(P0.01),NO content increased(P0.01),ΔΨm of cardiomyocytes increased(P0.05)and apoptosis rate decreased(P0.05).Compared with AI(90 g/L)group,after treatment combining with L-NAME,LDH activity and ROS content increased(P0.01),NO content decreased(P0.01),ΔΨm of cardiomyocytes decreased(P0.05)and apoptosis rate increased(P0.05).ConclusionAI can protect cardiomyocytes from H2O2 injury by increasing NO content and inhibiting cardiomyocyte apoptosis induced by ROS.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 2
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