Title: High expression of the chimeric antibody Fab against-CD_(20) in E. coli and bioactivity determination
Abstract: Amplify the gene of the variable region of heavy chain (V_(H)) and light chain (V_(L)) of the antibody against CD_(20) from the anti-CD_(20) single chain Fv antibody fragment (ScFv) expression vector by PCR method, clone the V_(H), V_(L) into the plasmid pYZF to construct the Fab expression vector pYZF1cd20, transformate 27C7 with pYZF1cd20, and the expression of Fab in 27C7. The CD_(20) binding Fab was expressed, isolated and purified, the competitive inhibition of immune fluorescence experiment show that the Fab inhibits the binding of monoclonal antibody HI_(47) to CD_(20) on the surface of Raji cell.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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