Title: Optimization of the mixed fermentation conditions for chitinase production and some properties of the crude enzyme
Abstract: As an original chitinase-producing strain s and strain d,screening from the soil,the study shows the mixed fermentation we get is a higher chitinase-producing.The optimal compositions of the medium for the strain producing chitinase are as follows(×10~(-2) g/mL):CO(NH_2)_2 1.0,K_2 HPO_4 0.07,KH_2PO_40.03, MgSO_4 7H_2O 0.05,yeast extract 0.5 and the chitin 1.0 (pH 5.5).The optimal cultivation conditions for the strain producing chitinase were 500 mL flask containing 100mL medium,2% amount of inoculation, 32℃incubating temperature and shaking cultivation at 130 r/min for 72hours.The most activity of chitinase of fermentation broth is 1.19 U/mL.Acorrding to the study,the crude enzyme showed a good stability.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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