Title: The cDNA cloning and sequencing Analysis of chicken β-defensin(Gal-8)
Abstract: In this study,the total cellular RNA was purified from chicken liver.According to the similarity gene sequence of birds in GenBank,two pairs of primers were designed.Then a 201bp chicken β-defensin-8(Gal-8) cDNA fragment was amplified by reverse transcriptionpoly-merase chain reaction(RT-PCR).Analysis of the sequence data showed that the chickenβ-de-fensin gene(the cDNA)fragment code 66 amino acids which comprised of signal peptide with 20 amino acid residues,original fragment with five amino acid and mature peptide with 41 amino acid residues.BLAST analysis showed that there was two bases difference in gene sequence of birds in GENBANK,the homogeneity of nucleotide was 99 %,The gene was ligated with plasmid pGEM-T Easy,Then the recombinant plasmid was transformed into E.coli DH5α,and the se-quence was identified by T/A clone.The mature peptide which was amplified from recombinant plasmid is 123 bp.This study provide an experimental basis for constructing recombinant nuclear protein and expressing defensins with a wide range of antimicrobial activities in the future.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot