Title: Cloning of aerolysin gene of Aeromonas trota from Eriocheir sinensis and rapid detection by PCR
Abstract: Synthetic oligonucleotide primers were used to clone aerolysin gene of Aeromonas trota from Eriocheir sinensis by polymerase chain reaction(PCR) technique.A DNA fragment of 622 bp was amplified from A.trota isolated from Eriocheir sinensis.The fragment was digested with BamHI restriction endonuclease and produced 570 bp and 50 bp fragments.This paper also studied PCR technique for the rapid detection of A.trota isolated from E.sinensis.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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