Title: Cloning and Bioinformatics of a New Gene,cry1Ab17 from Bacillus thuringiensis
Abstract:A new gene, cry1Ab17 was cloned from Bacillus thuringiensis WB9 by PCR with pMD18-T vector. Nucleotide sequences of cry1Ab17 were registered in GenBank with accession No. AY646166. It consists of 3 47...A new gene, cry1Ab17 was cloned from Bacillus thuringiensis WB9 by PCR with pMD18-T vector. Nucleotide sequences of cry1Ab17 were registered in GenBank with accession No. AY646166. It consists of 3 471 bases, which encoded a protein of 1 156 amino acids residues with 30.8% of hydrophilic amino acids, 45.2% of hydrophobic amino acids, 12.9% of acidic amino acids and 11.1% of basic amino acids. The deduced amino acid sequence of Cry1Ab17 showed 95.4% to 99.7% identity with those of the known Cry1Ab proteins. Four residues (Pro_ 170, Gly_ 449, Gly_ 796 and Gly_ 863) of Cry1Ab17 were found different from those of the respective corresponding position in all known Cry1Ab proteins. On the basis of the multiple alignment of the nucleotide and amino acid sequences of Cry1Aa, Cry1Ac, Cry1Ad, Cry1Ae, Cry1Ag and Cry1Ab, a phylogenetic tree was constructed using PAUP 4.0. SignalP analysis revealed that no signal peptide was found in Cry1Ab17. In addition, the secondary structure and three domains of Cry1Ab17 were also predicted and analysed. Fig 4, Tab 2, Ref 15Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 1
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