Title: Determination of the Content of Tetrahydropalmatine in Shugan Pills with HPLC
Abstract: Objective To set up a new method for the determination of the content of tetrahydropalmatine in shugan pills with HPLC. Methods The chromatographic conditions: A Spherisorb C_(18)(4.6 mm×250 mm,5 μm) served as the chromatographic column;methanolwater(75∶25,the pH value adjusted to 8 with 0.05 mol·L~(-1) Na_(2)HPO_(4) solution) was the mobile phase,the mixture was filtered through a Millipore membrane filter with an average pore diameter of 0.45 μm;UV detection wavelength,280 nm;column temperature,25 ℃;flow rate,0.9 mL·min~(-1). Results There was a good linear relationship between the corresponding peak areas and tetrahydropalmatine when its concentrations were in the range of 1.76-21.12 mg·L~(-1)(r=0.999 8).The average rate of recovery was 98.6% with an RSD of 1.38%. Conclusion The method was shown to be handy,accurate and reproducible and did not interfere with other medicinal materials in the prescription.It may thus be used as one of the methods for the content determination of tetrahydropalmitine in shugan pills.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot