Title: Determination of Rutin and Hyperoside in Extract from Crataegus pinnatifida Bge. by HPLC
Abstract: Objective To establish an HPLC method for Determination of hyperoside and rutin in the extract from Grataegus pinnatifida Bge.Method HPLC was used in the quantitative analysis by using Kromasil Cis(4.6mm×250mm,7μm)chromatography column and methanol-0.4% phosphoric acid(30:70) as a mobile phase.The flow rate of mobile phase was 1.0 mL/rain.The detection wavelength was at 359nm.Result The linear ranges for rutin and hyperoside were 0.051-0.51μg(r=0.9999),0.062-0.62μg(r=0.9997),respectively.The average recoveries were 100.4%(RSD=1.2%),99.0%(RSD=1.8%),respectively.Conclusion This method is easy,sensitive,reliable,accurate,reproducible and can be used to determine the contents of hyperoside and rutin in the extract from Grataegus pinnatifida Bge..
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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