Title: Determination of Baicalin in Jiuwei Qingre Jiaonang by HPLC
Abstract: Objective To establish a method for the determination of Baicalin in Jiuwei Qingre Jiaonang.Methods HPLC was used with DiamonsilC18(4.6mm×250mm,5μm) as chromatographic column and methanol-water-phosphoric acid(40:60:0.2) as mobile phase,the flow rate was 1.0ml/min and the detective wavelength was at 280nm.Results There was good linearity in range of 0.1384~1.384μg,(r=0.9997).The average recovery was 100.4% and RSD was 0.96%.Conclusion This method is sensitive,accurate,reproducible and provides a basis for the quality control of Jiuwei Qingre Jiaonang.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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