Title: Optimization of Agrobacterium-mediated Transformation Method for Rice
Abstract: [Objective] This study aimed to optimize the method for Agrobacterium-mediated transformation in rice.[Method] Transgenic rice seedlings were obtained by optimizing and modifying the classical transformation methods.[Result] Mature embryos were adopted and sterilized by HgCl2.Transformation efficiency had achieved the maximum when OD600 was 0.10,which had increased by 23%,comparing with previous method.[Conclusion] The study provided a technical reference for relative researches of Agrobacterium-mediated transformation in rice.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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