Title: Research on the expression level of transforming growth factor-β_1 in blood of silicosis patients
Abstract:To explore the relationship between TGF-β1 and silicosis,especially the possibility that TGF-β1 expression level in blood to be a available clinical index for silicosis disgnosis.16 silicosis patients...To explore the relationship between TGF-β1 and silicosis,especially the possibility that TGF-β1 expression level in blood to be a available clinical index for silicosis disgnosis.16 silicosis patients and 15 controls were selected as the objects of this study.8 ml blood were taken from those people for experimental detecting.Enzyme linked immunosorbent assay(ELISA) was used for the detection of serum TGF-β1 level,the expression level of TGF-β1-mRNA and TGF-β1 protein in peripheral lymphocytes were detected by RT-PCR and Western-blotting methods respectively.SPSS14.1 was applied for statistical analysis.The result showed that the serum expression level of TGF-β1 of silicosis patients was(203.14±32.38)μg/ml,significantly higher than that of controls(42.66±15.50) μg/ml(P0.01).The RT-PCR and Western-blotting detecting results also showed that the lymphocytes TGF-β1-mRNA expression level in silicosis patients was also obviously decreased compared with the controls.It indicated that serum TGF-β1 level is closely related to silicosis,suggests that it could be taken as a clinical index for the diagnosis of silicosis.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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