Title: Development of monoclonal antibodies against the P protein of rabies virus.
Abstract: The fusion protein(pET32a(+)-P)was purified by TrapFF crude,6-8-week-old BALB/c mice were immunized with the protein mixed with adjuvants before the splenic cells of immunized mice were fused with SP2/0 myeloma cells.Indirect fluorescence antibody detection assay was used to screen hybridoma cells for the specific monoclonal antibodies.One hybridoma(5G8) producing monoclonal antibody were obtained after three rounds of subcloning.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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