Title: Protection of Serum Containing Breviscapine on Cultured Hippocampal Neuron Injury Induced by Glutamate
Abstract: Objective: To investigate the protective effects and mechanism of Breviscapine on cultured rats hippocampal neuron injury induced by glutamate in the cell level. Methods: The drug-contained serum were obtained by the Seropharmacological method. When the primary hippocampal neurons of rat cerebral were cultured for 10 days, the mode were builded in which hippocampal neurons were exposed to 500μmol·L-1 Glutamate acid (Glu)for 20 minutes and the 5% drug-contained serum including normal, model, 0.05 g·kg-1 Nimodipine (Nim), 0.20 g·kg-1 Breviscapine and 0.05g·kg-1 Breviscapine were added to the nutrient solution of cultured neurons. In this study, the viability of cultured primary hippocampal neurons were detected by MTT assay, and the release of lactate dehydrogenase (LDH), malonydiadehyde (MDA) content and Nitric oxide(NO)content, the activity of Nitric oxide synthase(NOS)and Induced nitric oxide synthase (iNOS)were also detected. Results: The results showed that, when the hippocampal neurons were exposed to Glu, the viability of cultured primary hippocampal neurons decreased dramatically, the release of LDH, MDA content, Nitric oxide(NO)content, the activity of Nitric oxide synthase(NOS)and Induced nitric oxide synthase(iNOS)increased obviously compared with control. The Breviscapine could decreased the release of LDH, MDA content, Nitric oxide(NO)content, the activity of Nitric oxide synthase(NOS) induced nitric oxide synthase (iNOS). Conclusions: Breviscapine has protective effects on glutamate excitotoxicity of cultured primary hippocampal neurons of rats, and the mechanism of which may be related with improving energy metabolism, stabilizing cellular membrane, anti-liqid-peroxidation, reducing NO content, the activity of NOS and iNOS.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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