Title: Effect of Bufalin on Inducing Cell Apoptosis and Its Mechanisms in Human Lung Adenocarcinoma Cells
Abstract: Objective To study the mechanism of bufalin on inducing cell apoptosis in human lung adenocarcinoma(A549)cells.Methods The growth inhibition was analyzed by MTT assay;The morphological changes in the samples dyed in wright-Giemsa were observed using light microscope.Apoptosis and Mitochondrial membrane potential(ΔΨm)was evaluated by flow cytometry,Western blot analysis was used to evaluate the level of Bcl-2 and Caspase-3 expression.Results (1)Bufalin inhibits cell growth,and 50%inhibitory concentrations(IC50)of the agent at 48h and 72h were(56.14± 6.72)nmol/L,(15.57±4.28)nmol/L,respectively.(2)Apoptosis of A549 cells could be efficiently induced by bufalin at different concentrations.(3)A significantly reduction in the Mitochondrial membrane potential(ΔΨm)was induced by bufalin.(4)The expression of Bcl-2 protein was downregulated and Caspase-3 protein was activated during bufalin-induced apoptosis in A549 cells.Conclusion Bufalin-induced lung adenocarcinoma cells apoptosis is through mitochondria signaling pathway in A549 cells.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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