Title: Protective effect of allitridi against cerebral ischemia-reperfusion injury in rat hippocampus
Abstract: AIM To explore the relation of the protective effect of allitridi against cerebral ischemia-reperfusion injury and the Ca2+ transporting mechanism in hippocampal neurons of rats.METHODS The global cerebral ischemia-reperfusion models were established by 4-vessel occlusion.Allitridi 10,20 and 30 mg·kg-1 was injected through rat tail vein,half dose at 30 min before brain ischemia and another half dose at 10 min after reperfusion,respectively.The hippocampus of rat was removed 24 h after reperfusion.Toluidine blue staining was used to investigate histological grade and survival neuronal density of hippocampal CA1 subfield by microscope.The Pi-colorimetric method was used to measure the activity of Ca2+-transporting ATPase.Atomic absorption was used for analysis of Ca2+ content in hippocampal tissue.RESULTS Ten minutes cerebral ischemia then reperfusion for 24 h resulted in distinguished morphological changes and the decrease in survival neuronal density of hippocampal CA1,and induced the depression in Ca2+-transporting ATP enzyme activity and increase in Ca2+ content of hippocampal tissue.Allitridi intravenous administration significantly lightened the damage in hippocampal tissue induced by cerebral ischemia-reperfusion and increased the survival neuronal density.At the same time,allitridi remarkably increased the Ca2+-transporting ATPase activity and reduced Ca2+ content of hippocampal tissues of ischemic-reperfused rats.CONCLUSION Reducing Ca2+ content in hippocampal neuron by increasing the activity of Ca2+-transporting ATPase maybe one of the protective mechanisms of allitridi against cerebral ischemia-reperfusion injury.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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