Title: Screening and Identification of High Saccharifying Rhizopus oryzae
Abstract:In order to screen a high saccharifying strain of rhizopus that is suitable for pure culture of glutinous rice wine,19 Rhizopus spp.strains isolated from different solid starters are examined for thei...In order to screen a high saccharifying strain of rhizopus that is suitable for pure culture of glutinous rice wine,19 Rhizopus spp.strains isolated from different solid starters are examined for their high ability to saccharify starch.Rhizopus 8-3Mis selected as the functional Rhizopus strain with the highest reducing sugar[(64.4±0.6)g/100 g rice]and glucose production[(48.1±1.4)g/100 g rice]after cultured for 3days at 28 ℃.Based on morphological test and phylogenetic analysis of26 SrDNA D1/D2 region and internal transcribed spacer(ITS)sequences,8-3M is identified as Rhizopus oryzae.Then the enzyme activity testing and alcoholic fermentation testing produced by R.oryzae 8-3Mare studied.The highest amylase activity of crude enzyme solution from R.oryzae 8-3M in YPS broth for 4days at 28℃is(69.6±1.7)U/mL.R.oryzae 8-3Mcultured in cooked glutinous rice for 96 hat 28℃gives(36.8±2.0)U/mL of the highest glucosidase activity and(1.0±0.1)%(V/V)of ethanol content.Because of its ability of starch-degrading and small amount of ethanol fermentation,R.oryzae 8-3Mmay have a potential application in making defined fungal starter for glutinous rice wine.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 1
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