Title: Detection of plasma soluble HLA-G and lymphocyte subsets in peripheral blood of children with infectious mononucleosis
Abstract:OBJECTIVE To investigate the levels of plasma soluble HLA-G(sHLA-G) and the lymphocyte subsets in peripheral blood,with the aim to explore the relationship between plasma soluble HLA-G and the lymphoc...OBJECTIVE To investigate the levels of plasma soluble HLA-G(sHLA-G) and the lymphocyte subsets in peripheral blood,with the aim to explore the relationship between plasma soluble HLA-G and the lymphocyte subsets in peripheral blood of children with infectious mononucleosis(IM) caused by EB virus.METHODS We determined the plasma sHLA-G levels of 51 children with EBV infectious mononucleosis and 146 healthy children by enzyme linked immunosorbent assay(ELISA),and the lymphocyte subsets in peripheral blood of 86 children with EBV infectious mononucleosis and 30 healthy children were analyzed by Flow Cytometry(FCM).RESULTS The plasma sHLA-G level of the children with EBV infectious mononucleosis was 130.30(77.28~217.23)U/ml,which was significantly higher than that of the control group 19.82(6.39~44.90)U/ml,and statistically significant difference was observed between the two groups by the Mann-Whitney U test(Z=-9.472,P0.01).The percentage of CD3+ and CD8+ cell populations significantly increased in the children with EBV infectious mononucleosis as compared with the control group(P0.01).However,the percentage of CD4+,CD4+/CD8+,CD16+56+,CD19+ cells markedly decreased in the children with EBV infectious mononucleosis as compared with those in control group.The differences were statistically significant(all P0.01).None of the changes of CD3+,CD4+,CD8+,CD4+/CD8+,CD16+56+,CD19+ cell populations in the children with EBV infectious mononucleosis was significantly related to the level of plasma sHLA-G.CONCLUSION The levels of plasma sHLA-G significantly increase and the cellular immunity is in great disorder in the EBV infectious mononucleosis children.The level of plasma sHLA-G can be considered as an index for auxiliary diagnosis of EBV infectious mononucleosis,and further studies are needed to explore the mechanism of the increase.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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