Title: Cloning and analyzing the function of the gene of human phage antibodies with single - chain variable fragment specific to hepatocellular cancer
Abstract: Objective Cloning and characterizing the gene of human phage antibodies with single - chain variable fragment (ScFv) specific to hepatocellular cancer. Methods The recombinant phages inserted with ScFv were determined by PCR.The recombinant phages were panned by human hepatocellular cancer cell line of SMMC - 7721. After 4 rounds of biopanning, the specificity of ScFv from the monoclonal bacteria was determined by ELBA. Results The rate of recombinant bacteria inserted with ScFv was 70% . From the 1st round to the 4th round of the panning, the number of the eluted phages had increased 381 times. Conclusion: Acquired phage antibodies with ScFv specifically combined with hepatocellular cancer by the phage antibody panning.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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