Abstract: According to the Principle of shot gun, rabbit genoinic library is constructed.To cope with the method of isotope labeling used in screening, library usingbacteriophage (EMBL3) is constructed. It carries (each recombinane) rabbit genomicDNA about 20kb, and 10 ̄6 recombinants are collected.Using hGH cDNA as probe, the library is screened and verified by means of insitu hybridization of bacteriophage plaques, dot blot and Southern blot. The resultsshow that, firstly, there are homologous regions between hGH gene and rabbit GH gene,and identification of rabbit GH gene with hGH cDNA is workable; Secondly, the reco-mbinant clones carrying rabbit GH gene are in the library.This study lays the foundation for research on the nearly unknown rabbit GHgene.
Publication Year: 1994
Publication Date: 1994-01-01
Language: en
Type: article
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