Title: The effects of two-step sequential therapy on signal pathway of NF-κB on airway inflammation in asthmatic mice
Abstract: Objective To study the effects of two-step sequential therapy on signal pathway of NF-κB of airway inflammation in asthmatic mice.Methods Forty healthy female Kunming mice were divided into four groups randomly(n=10,in each group):normal control group,asthma group,budesonide group and two-step sequential therapy group.The asthmatic models were established by ovalbumin,and budesonide and two-step sequential therapy were applied to them.Then the pathologic changes in airway tissues and bronchoalveolar lavage fluid(BALF)in cytology were observed.The level of nuclear factor-κB(NF-κB)and GATA-3 in airway tissues was detected by immunohistochemical method.The level of interleukin-4(IL-4)in BALF was detected by enzyme-linked immunosorbent assay(ELISA).Results The treatment groups had less inflammatory cell infiltration than asthmatic group(P0.01),and more than normal control group(P0.01).There was no significant difference between the budesonide group and two-step sequential therapy group(P0.05).The level of NF-κB,GATA-3 and IL-4 decreased in the budesonide group and two-step sequential therapy group compared with the asthmatic group(P0.01),but increased compared with the normal control group(P0.01).There was no significant difference between budesonide group and two-step sequential therapy group(P0.05).Conclusions The two-step sequential therapy may inhibit the activity of the signal transduction pathway of NF-κB,which can reduce the expression of GATA-3 and class cytokines of Th2,hence plays an important role in treating asthmatic mice.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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