Title: Inhibitory effect of total ginsenosides on cardiomyocyte hypertrophy induced by angiotensinII in neonatal rats
Abstract: OBJECTIVE To observe the effect of total ginsenosides(TG) on angiotensinⅡ(AngⅡ)-induced cardiomyocyte hypertrophy and its possible mechanisms.METHODS After 3 d,the cultured primary cardiomyocytes were randomly divided into normal control group,model group with AngⅡ 0.1 μmol·L-1 and TG treated groups(AngⅡ+TG 50,100 and 200 mg·L-1,respectively) for 48 h,respectively.The cell diameter was measured by HE staining,and protein content was determined by Bradford method.The intracellular free Ca2+ concentration(i) was assayed by fluorescent determination using Fura-2/AM.The mRNA expression of atrial natriurertic factor(ANF),calcineurin(CaN) and extracellular signal-regulated kinase 1(ERK1) was assayed by real time PCR.The protein expression of α-subunit of CaN(CnA) and mitogen-activated protein kinase phosphatase1(MKP1) was assayed by Western blotting.RESULTS Cell diameter was increased from(32±8)μm to(79±17)μm,protein content per cell from(416±9)pg to(541±16)pg,and ANF mRNA expression from 34±5 to 268±36 by AngⅡ.i and mRNA expressions of CaN and ERK1 were elevated,so was the protein expression of CnA by AngⅡ.However,they were reversed by TG 50,100 and 200 mg·L-1 in a concentration-dependent manner.The cell diameter was reduced by 15.3%,37.7% and 51.2%,and protein content by 4.0%,15.6% and 19.4% by TG 50,100 and 200 mg·L-1,respectively.In addition,TG could also increase the MKP1 protein expression compared with AngⅡ treated-group.CONCLUSION TG can inhibit the AngⅡ-induced cardiomyocyte hypertrophy,which is related to its inhibition on CaN and ERK signalings.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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