Title: Effects of sodium ferulate on hepatocyte growth inhibited by transforming growth factor β_1
Abstract: AIM To research effects of sodium ferulate(SF) on transforming growth factor β_1 inhibited hepatocyte growth. METHODS Human cultured hepatocytes(L02 cells) served as control group ,TGFβ_1(5 μg·L -1 ) was used to inhibited L02 cells to construct the cell model. MTT methods were used to study the growth inhibitory effect of TGFβ_1 on L02 cells. DNA synthesis was analyzed by measuring 3H-TdR incorporation. The effects of SF on the changes of cell cycle were analyzed by flow cytometry. Generation of intracellular ROS was determined by the emission of fluorescence in L02 cells proloaded with DCFH-DA fluoresin and then treated them with TGFβ_1 or SF. After 4h,control, TGFβ_1 and TGFβ_1+SF-treated cells were analyzed by flow cytometry. RESULTS ①Incubated in the presence of TGFβ_1 for 24 h, 67.93% of L02 cells survived (P0.05 vs control).250 μmol·L -1 of SF increased cell viability by 19.28% (P0.05 vs treatment),and the 3H-TdR incorporation reduced by TGFβ_1 by 17.71% (P0.05 vs TGFβ_1 group). ② 125 μmol·L -1 and 250 μmol·L -1 of SF increased the percentage of cells in S phase by 11.45% and 17.92% (P0.01 vs control), in G_2 phase by 3.29% and 7.85% respectively(P0.05 vs control). ③ By treating L02 cells with TGFβ_1 for 4 h, the level of intracellular peroxide was 1.90-fold compared with that of untreated cells. 62.5 μmol·L -1 ~250 μmol·L -1 SF obviously decreased fluorescence by 14.5% to 27.5% respectively. CONCLUSION SF could reduce TGFβ_1 induced growth arrest of L02 cells, which may be associated with the antioxidant effect and the promotion of the cell cycle processes from G_1 phase to S phase.-
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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