Title: Effects of Acupuncture-rehabilitation Method on Behavior and Expression of GAP-43 and Nogo-A in Ischemic Cerebral Cortex in Rats after Focal Cerebral Ischemia
Abstract: Objective:To explore the effects of acupuncture-rehabilitation method on behavior and the expression of GAP-43 and Nogo-A in the cerebral cortex in rats after focal cerebral ischemia.Methods:A total of90 SD rats were randomly divided into a model group,a sham operation group,an acupuncture with rehabilitation group,a rehabilitation group and an acupuncture group.Each group was subdivided into 3 subgroups by 3 d,7d,14 d.The acupuncture-rehabilitation method was used in the acupuncture with rehabilitation group;treadmill running was trained in the rehabilitation group;only the cluster needling of scalp point was punctured in the acupuncture group;the sham operation group and the model group had no intervention.Behavioral test of rats was evaluated by net screen test,and immunohistochemistry staining was used to observe the expression of GAP-43 and Nogo-A in ischemic cortex in rats in each group at different time points.Results:After 7 d and14 d,compared with the rehabilitation group and acupuncture group,the screen test score in acupuncture-rehabilitation group was decreased obviously,and the difference was statistically significant( P 0.05);after 7 d and 14 d,the expression of GAP-43 positive cells in ischemic cortex was higher in acupuncture-rehabilitation group than that in the model group,rehabilitation group and acupuncture group( P 0.05);after 14 d,the expression of Nogo-A positive cells in acupuncture-rehabilitation group in ischemic cortex was lower than that in the model group,rehabilitation group and acupuncture group,and the difference was statistically significant( P 0.05).Conclusion:The acupuncture-rehabilitation method can promote recovery of behavior in rats after focal cerebral ischemia and the mechanism may be related to increasing the expression of GAP-43 in cortex and decreasing the expression of Nogo-A in cortex.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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