Title: Optimization of fermentation conditions of lactic acid from starch by Bacillus licheniformis
Abstract: The fermentation conditions of lactic acid from starch by Bacillus licheniformis were investigated.A lactic acid yielding strain Bacillus licheniformis WX51 was isolated from the soil of suburb of Beijing.By single factor experiments and orthogonal tests,it was found that the optimum fermentation medium contained 60 g/L soluble starch,0.5 g/L(NH4)2SO4,1.36 g/L KH2PO4,0.2 g/L MgSO4.7H2O,0.01 g/L FeSO4.7H2O,2 g/L NaCl,0.5g/L corn syrup and 20 g/L CaCO3.The optimum fermentation conditions were 250 mL flask filled with 10 % medium,50℃,200 r/min and cultured for 40 hours,and the inoculum was 2 %.After optimization,the production of lactic acid from soluble starch increased from 28.4g/L with a conversion of 71.0% to 36.5 g/L with a conversion of 91.2 %;the volumetric production rate increased from 0.6 g/(L.h) to 0.9 g/(L.h).
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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