Title: Isolation and Sequence Analysis of Chitinase Gene LeCHI from Lycopersicum esculentum
Abstract: PCR primers were designed according to known sequences in GenBank.A gene,designated LeCHI,was isolated from leaf cDNA of tomato(Lycopersicum esculentum).Accession number in NCBI GenBank is FJ849060.The coding sequence of LeCHI is 762 bp in length encoding 253 amino acids.RT-PCR results show that the LeCHI gene is expressed in roots,stems,leaves and flower buds.Sequence comparison results show that LeCHI has high sequence homology with those of Solanum.tuberosum and Capsicum.annuum.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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