Title: A STUDY ON THE APOPTOSIS OF HUMAN HEPATOMA CELL LINE HepG2 INDUCED BY MATRINE WITH FLOW CYTOMETRY
Abstract: Objective To study the function of matrine in inhibiting proliferation of HepG2.Methods The cells were treated with 0~2 0 g/L matrine for 24~72 h,marked by PI,and with 0~3 0 g/L matrine for 24~72 h,marked by AnnexinV FITC /PI.Cell apoptosis was assayed by FCM.Results HepG2 apoptosis peak appeared after they were treated by 0 8,1 5,2 0 g/L matrine for 72 h,the percentage fo apoptosis cells were 10 57%,16 37%,and 10 85% respectively.Early stage apoptosis examination:when the cells were treated with 2 0,1 5,3 0 g/L matrine for 6,12,12 h,their percentages of apoptosis cells were 7 7,8 6,15 7% respectively.At the same time,nercrosis cells were increased,when the cells were treated with 3 0 g/L matrine for 12 h,necrosis cells rose to 21 9%.Conclusion Matrine in certain concentrations can induce HepG2 cells apoptosis and necrosis co exist, depending on matrine's affecting dosages and time.$$$$
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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Cited By Count: 1
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