Title: Optimizing Extraction Conditions for Total Flavonoids in Hypecoum leptocarpum with Orthogonal Test
Abstract: [Objective] To study the optimal extraction technique for total flavonoids in Hypecoum leptocarpum and determine its content. [Method]With total flavonoids content as investigation index,methy alcohol concentration,solid-liquid ratio and extraction time as investigation factors,the optimum conditions for extracting total flavonoids were determined by orthogonal test. [Result] The optimum conditions for extracting the total flavonoids by reflux are as following: material-liquid ratio: 1∶15,50% methy alcohol,ultrasound extraction 2 times and 20 min for each time. Under the above conditions,the highest content of total flavonoids in H. leptocarpum is 48. 63 mg / g. [Conclusion] The method is easy operation and its result is accurate,extraction efficiency is higher and can be used for study on H. leptocarpum.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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