Title: Effect of MMP-2 Antisense Oligodeoxynucleotide on Proliferation and Invasion of Human Gastric Cancer SGC7901 Cells
Abstract: Objective To investigate the effect of matrix metalloproteinase-2(MMP-2)antisense oligodeoxynucleotide (ASODN)on the proliferation and invasion of human gastric cancer SGC7901 cells. Methods Various concentrations of thiophsphorylate-modified ASODN of MMP-2 were transfected to moderately and lowly differential SGC7901 cells. The transcription level of MMP-2 mRNA was determined by RT-PCR, the abilities of in vitro migration and invasion of cells by Transwell test, and the proliferative activity of cells by MTT method. Results The transcription level of MMP-2 mRNA in SGC7901 cells after transfection with ASODN decreased significantly. Both in vitro migration and invasion abilities of cells were inhibited, and the inhibitory effect increased with the increasing ASODN concentration. However, the proliferative activity of cells showed no significant change. Conclusion MMP-2 ASODN inhibited the transcription level of MMP-2 mRNA as well as in vitro migration and invasion, while showed no significant correlation to the proliferative activity of SGC7901 cells.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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