Title: Cloning and immunohisto-localization of the FKBP12 gene of Schistosoma japonicum
Abstract: The cDNA of the FKBP12 gene of Schistosoma japonicum(Sj) was cloned and its immunohisto-loacalization was investigated in the present study,in which the full length of this gene was obtained from the adult worm RNA of S.japonicum by RT-PCR, and then was cloned into vectors of pGEX-4T-1 and pEGFP-N1, and indirect immunofluorescence assay was used to find its histo-localization in various tissues. It was demonstrated that the FKBP12 gene cDNA sequence could be cloned successfully into vectors pGEX-4T-1 and pEGFP-N1,and localized on the tugment of S.japonicum. The result of this investigation provides basis for the further studies on vaccine development.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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