Title: Generation Mechanism of A Semi-dwarf Mutant and A Lethal Yellow Seedling Mutant in A Rice Segregating Progeny Lines
Abstract: [Objective] The study aimed to identify the Ds-tagged genes of the mutants sdwrky and lysrcc1,and to analyze their molecular mechanism.[Method] The Ds-flanking sequences were cloned from the two mutants by using TAIL-PCR amplification technique.[Result] Analysis of the Ds-flanking sequences indicated that one Ds element was inserted into the Os03g0599600 gene(sdwrky)of rice chromosome 4 in the sdwrky mutant;Another Ds element was inserted into the Os04g0597300 gene(lysrcc1)of rice chromosome 3 in the lysrcc1 mutant.[Conclusion] The sdwrky gene encoded a WRKY domain containing protein.SDWRKY probably had the similar function of OsWRKY24 which was a negative regulator encoded by a rice WRKY gene repressing both ABA and GA signaling in aleurone cells.The sdwrky gene mutation resulted in the sdwrky mutant phenotypes.The lysrcc1 gene encoded a regulator of chromosome condensation(RCC1)repeat containing protein(LYSRCC1).As a Ran GEF,YLRCC1 probably played an important role in the regulation of nucleocytoplasmic transport,which was essential for chloroplast synthesis.The lysrcc1 mutant was probably caused by a single recessive mutation of the lysrcc1 gene.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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