Title: [Determination of ellagic acid compounds in Euscaphis japonica by using UPLC].
Abstract: To develop an ultra performance liquid chromatography (UPLC) method for simultaneous determination of five ellagic acids in Euscaphis japonica.Analysis was carried out on an Acquity BEH C18 (2.1 mm x 100 mm, 1.7 microm) column at 40 degrees C eluted with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.2 mL x min(-1), and the detection wavelength set was monitored at 245 nm.All calibration curves showed good linear relationship within test ranges (r >0.9997), and the overall recoveries were in the range of 97.2%-102.1%, with RSD less than 3.2% (n = 6). The overall RSD of precision test were less than 2.9%.The developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of E. japonica.
Publication Year: 2009
Publication Date: 2009-12-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 1
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