Title: Effects of CD28 superagonist antibody on immune inflammatory reaction early after orthotopic tracheal transplantation in rats
Abstract: Objective:To investigate the effects of superagonistic CD28-specific monoclonal antibody JJ316(supCD28 MAb) on in vivo proliferation of rat CD4+CD25+Fox P3+ Treg(Treg) cells and on allograft rejection reaction in a rat orthotopic tracheal transplantation model.Methods:Rat orthotopic tracheal transplantation models were divided into two groups in the present study.The experimental group was treated with supCD28 MAb(0.5 mg/rat) via intraperitoneal injection on the day of transplantation.Control group was injected with mIgG(0.5 mg/rat).The proportions of CD4+CD25+ FoxP3+ T cell population in cervical lymph nodes,spleen and peripheral blood monocytes were examined by flow cytometry 5 days after operation.The tracheas were also harvested for histological evaluation.Results:The allografts of the experimental group showed greatly improved airway obliteration,infiltration of inflammatory cells,and respiratory epithelial injury compared with those of the control group.Furthermore,The experimental group had significantly increased CD4+CD25+ FoxP3+ Treg cell population in the lymph nodes,spleen and peripheral blood monocytes compared with those in the supCD28 MAb group([5.8±1.2]% vs [16.9±4.2]%,[14.8±3.6]%,and [2.9±0.9]%,[3.3±1.3]% vs [2.8±1.4]%,respectively,P0.05).Conclusion:SupCD28 MAb can attenuate airway inflammation injury after orthotopic tracheal transplantation.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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