Title: Effects of Propofol on Embryonic-and α7-Nicotinic Acetylcholine Receptors
Abstract:Objective:To investigate the effect of propofol on embryonic-and α7-nicotinic acetylcholine receptors with whole cell clamp patch.Methods:The embryonic-and α7-nicotinic acetylcholine receptors were he...Objective:To investigate the effect of propofol on embryonic-and α7-nicotinic acetylcholine receptors with whole cell clamp patch.Methods:The embryonic-and α7-nicotinic acetylcholine receptors were heterologously expressed in HEK293 cells and activated to maximal currents by the application of acetylcholine(100 μmol/L).Peak currents were recorded in HEK293 cells treated by propofol using whole cell patch clamp technique.Results:Propofol produced rapid and readily reversible,concentration-dependent inhibition.The inhibitory effect of propofol was greater to embryonic-nicotinic acetylcholine receptor compared to that of α7-nicotinic acetylcholine receptor(P 0.05).Propofol wasn't resulted in a concentration-dependent inhibition in embryonic-and α7-nicotinic acetylcholine receptors(P 0.05).Conclusion:There was inhibitory effect of propofol on embryonic-and α7-nicolinic acetycholine receptors,which was independent of the concentration of propofol.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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