Title: Establishment of gene sequencing method for detecting severe fever with thrombocytopenia syndrome bunyavirus in Anhui province
Abstract:Objective To establish the gene sequencing method for detecting severe fever with thrombocytopenia syndrome bunyavirus(SFTSV),and to appraise the ten suspected virus strains from patient serum by anal...Objective To establish the gene sequencing method for detecting severe fever with thrombocytopenia syndrome bunyavirus(SFTSV),and to appraise the ten suspected virus strains from patient serum by analysis of its sequences in Anhui province in 2011.Methods Primers were designed,and the viral nucleic acid was amplified by RT-PCR method,and the product of the amplification DNA was sequenced.Results The three pairs of primers would be efficient amplification of nucleic acid sequences.The ten suspected virus strains were similar to SFTSV above 93%,and more closed related to Phlebovirus AH12 and Phlebovirus HN6,less related with other Phlebovirus.Conclusion The ten virus strains isolated from patient serum were severe fever with thrombocytopenia syndrome bunyavirus in 2011.So we should strengthen the disease monitoring in Anhui province,and make the effective prevention of itsspread.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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