Title: Secondary infection of neuroblastoma cells by poliovirus induces cytopathic effect
Abstract:Objective: Poliovirus is the etiologic agent of poliomyelitis. The initial event of poliovirus infection is the attachment of the virion to poliovirus receptor (PVR), which is found only on the surfac...Objective: Poliovirus is the etiologic agent of poliomyelitis. The initial event of poliovirus infection is the attachment of the virion to poliovirus receptor (PVR), which is found only on the surfaces of primate cells. The productive infection of permissive cells with poliovirus induces dramatic morphological alterations termed cytopathic effect. Methods: Human neuroblastoma cells (SK-N-SH) infected with type I poliovirus Mahoney strain (PV1/Mahoney) displayed typical signs of CPE at 4 hours post-infection. Analysis of the kinetics of virus production from PV1/Mahoney-infected SK-N-SH cells demonstrated that the newly synthesized viruses appeared at 4 hours post-infection. Results: Treatment with specific antibodies against PV1/Mahoney or PVR within 4 hours post-infection protected most of the infected cells from development of cytopathic effect. Before addition of antibodies, re-infection of the infected cells with wild type viruses induced CPE and cell death. However, before antibody treatment re-infection of the infected cells with the inac-tivated polioviruses significantly reduced virus-induced cytopathic effect. Conclusion: Our results suggested that secondary infection of the cells was critical to the development of PV-induced cytopathic effect.Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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