Title: Changes of splenocyte lymphokines in ICR mice induced by recombinant Egmyophilin(rEgmyophilin)vaccine against Echinococcus granulosus
Abstract: To investigate the immunologic mechanism in mice immunized with recombinant Egmyophilin vaccine of E.granulosus and against challenge with Egprotoscolecs,ICR mice were subcutaneously and intranasally vaccinated by the vaccine,following by the challenge with Egprotoscolices on the 8th week of vaccination and killed on the 20th week of infection to obtai spleen.Spleen cells were separated and the proliferation was determined by MTT method.Then the cells were collected,stained by PI Annexin V-FITC.The supernatant was collected and measure the level of IL-2,IL-10,IFN-γ and TNF-α by ELISA,and the blank vector and PBS were served as control.As demonstrated by the MTT assay,the recombinant Egmyophilin vaccine could stimulate specifically the proliferation of splenic T lymphocytes in mice.ELISA assay indicated that the level of IL-2,IFN-γ and TNF-α in immunity group were significantly increased after stimulus with ConA or recombinant Egmyophilin.The results indicated that the recombinant Egmyophilin vaccination induces Th1 cellular response in the mice against the challenge of Egprotoscoleces.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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