Title: Effects of resveratrol on L-type calcium current (I_(Ca-L)) in guinea pig ventricular myocytes
Abstract:Aim To investigate effects of different concentrations of resveratrol (RES) on L-type calcium channels in guinea pig ventricular myocytes. Methods Enzyme digestion was used to isolate single ventricul...Aim To investigate effects of different concentrations of resveratrol (RES) on L-type calcium channels in guinea pig ventricular myocytes. Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record L-type calcium current. Results RES inhibited ICa-L in a concentration- dependent manner .The application of 1、10、100 μmol·L-1 RES reduced the density of peak ICa-L from ( -12.96±1.48 ) pA/pF to (-11.36±1.59)pA/pF (n= 6,P0.01), (-9.96±1.51) pA/pF (n= 6, P 0.01) and (-7.77±0.68) pA/pF (n= 6,P0.01) pA/pF at 0 mV, respectively.ICa-L came back to (-11.85±0.83) pA/pF after washout. RES remarkably shifted activation curve to the right, and delayed the voltage-dependent steady-state activation of calcium current. The half activation potential (V1/2) was ( -32.32801±0.366 )mV in the control and(-23.36414±0.94711)mV in the presence of RES 1 μmol·L-1. The slope factor(k) was (8.86352±0.37865) in the control and (8.7592±0.8847 ) in the presence of RES 1 μmol·L-1(n= 6,P0.05). Steady-state inactivation curve was not affected markedly, also V1/2 value and k value in the presence of RES 1 μmol·L-1 were not markedly different from those of the control. The recovery time from inactivation was not changed significantly. Conclusion RES decreased the amplitude of L-type calcium current, which would be one of cardiac protection mechanisms of the drug. And slowing the activation procession of ICa would help to its cardiac protection.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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