Title: A Study on RNAi-mediated Inhibition of survivin Gene on Proliferation of Human Esophageal Cancer EC109 Cells in Vivo
Abstract: Objective To investigate the RNA interference-mediated inhibition of survivin gene on the proliferation of EC109 cells in vivo.Methods siRNA-survivin eukaryotic expression vector was constructed to transfect EC109 cells which were further screened and evaluated to select stable cell clones.Meanwhile,non-specific siRNA vector transfection was prepared as control;Proliferation in vitro of three groups of cells were compared in terms of time of tumor formation and weight of tumor nodules on the model of implanted tumors in nude mice bearing esophageal cancer xenografts;Cell apoptosis of implanted tumors were detected by immunohistochemistry.Results Time of tumor formation of the group of siRNA-survivin transfected EC109 cells in nude mice was 8.8571±1.06904 days,longer than that of control groups;mean tumor weight of the experimental group was 0.3243±0.11998 g,lower than that of control groups,with a marked difference(P0.05);Cell apoptosis was detected by TUNEL(TdT-mediated-biotinylated-dUTP nick end labeling),with the finding that apoptosis index(AI) of the experimental group was 10.52±4.35,higher than that of control groups,with a marked difference(P0.05);Conclusions RNAi-mediated survivin gene can inhibit proliferation in vivo of human esophageal cancer EC109 cells and the major mechanism is induce cell apoptosis.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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