Title: Technique of extracting bioactive components in Dandelion
Abstract: Objective To develop a method for extraction and determination of bioactive components in Dandelion,such as chlorogenic acid,caffeic acid,coumaric acid,ferulic acid,and quercetin.Methods These bioactive components were exacted by 4 extraction methods(ethanol extraction,ethanol reflux,soxhlet extraction,and microwave extraction),and the factors of extracting solvents and extracting time were considered.The separation was performed on an Eclipse XDB-C18 column(150 mm×4.6 mm,5 μm) with linear gradient elution composed of methanol and 1% acetate acid solution at 30 ℃,and detected at each maximum absorption wavelength using diode array detector.The flow-rate was 1.0 mL·min-1.Results The method had good repeatability and linearity,and all the correlation coefficients were bigger than 0.999 6.Their detection limits were 10,4,5,8 and 20 μg·L-1(S/N=3),respectively.The optimal microwave extraction conditions were as follows: microwave output power was 500 W,extracting solvent was 40% ethanol/water(v/v),extracting time was 60 s.Conclusion The extraction method is efficient with short extraction time.The analytical method is sensitive,accurate and suitable for the determination of effective compounds in Dandelion.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 1
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