Title: Immobilization and Characteristics of Fungal Laccase on Polyethyleneaminated Chitosan
Abstract: Laccase has been immobilized on polyethyleneaminated chitosan with glutaraldehyde as crosslinking agent.When glutaraldehyde concentration is 2%,linking time is 14 h,immobilization time is 6 h and laccase dosage is 70 mg/g carrier the optimal condition was obtained.In this way 64 U/g of immobilized enzyme was available.The Km for the immobilized enzyme is 8.73 mmol·L-1 and the optimal pH and temperature for immobilized enzyme are 4.2 and 50 ℃.Compared with the free enzyme,its stability is considerable improved.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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